Gladstone Eyre (1862–1933) was an Australian portrait and landscape painter active mainly in Sydney and Launceston from the 1880s to the early 1900s. Born in Brunswick, Victoria, he trained first under Van den Houten and later under Knud Bull after moving to Sydney. During the 1880s he built a strong reputation as a portrait painter, producing works of leading public figures including church leaders, politicians and business figures.

Eyre became a member of the Art Society of New South Wales in 1883 and maintained a studio in George Street, Sydney. In 1891 he moved with his family to Launceston, where he painted portraits and Tasmanian landscapes in oils, watercolours and crayon, taught evening art classes, and restored older paintings.

Returning to Sydney in 1902, Eyre continued to paint widely across New South Wales, with particular interest in Sydney Harbour and the Blue Mountains. He remained active until his death in Sydney Harbour in 1933.